In creating viral vectors suitable for gene delivery to various cell types (ie. broader or narrower range of cells), pseudotyping is used to change the tropism of the virion. For instance, the envelope proteins from wild-type HIV-1 recognize and fuse to CD4, a co-receptor present on the surface of helper T cells.

However, the envelopes of viral vectors can be manipulated to provide different infection outcomes. Our Core uses vectors in which the HIV envelope protein is replaced with vesicular stomatitis virus glycoprotein-g (VSV-G). VSV-G is suitable for experiments involving widespread infection of cells because its phospholipid receptor is universally expressed in mammalian cells and thus, has a relatively broad tropism.

While a broad tropism may be more suitable for some experiments, others may require higher specificity for infection. The Core is here to advise and provide the resources for your specific needs. If you would like virus with a coat other than VSV-G, please contact us.