Here, we have compiled some sources links that may be helpful for your experiments with our virus preps. We are committed to providing you with the highest quality and titer of virus possible and are here to support you with your experiments.
Make the most out of your virus
Optimizing transduction efficiency
We provide you with superior quality and high titer lentivirus, however, transduction efficiency can vary among cell types. See the following publications which may help you to optimize your tranductions.
Denning et. al found that adding certain combinations of polycations and sera during infection significantly increased transduction efficiency in 293FT and H1080 cells. Such modifications may be worth exploring, depending on the infectivity of your particular cell lines.
Delville et. al found that a non-ionic reagent, LentiBoost, outperformed polycations at increasing transduction efficiency in murine cytotoxic T cells, helper T cells, and hematopoietic progenitor cells.
Alternatively, see Addgene's tips for troubleshooting viral transductions. Their blog provides insight into how viral vector stability, storage, and titer may affect transduction efficiency. They also highlight reagents, such as polybrene and fibronectin, which may help enhance transduction.
Storage
Another way to optimize experiments with your virus is to properly maintain (ie. store) your virus.
Rahman et. al investigated how different storage conditions (4°C versus -80°C) affect the morphology and titer of lentivirus. They found that storing virus at 4°C for 3 and 7 days led to a 60% and 30-40% reduction in titer, respectively. Storing virus at -80°C for one day led to only a slight decrease in titer. Therefore, we recommend that virus be stored at -80°C to maintain high titers for effective transduction.