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What titers should I expect for different sized vectors?

By vaidehi on August 18, 2020

 

Vector size (kb) Expected titer (TU/mL)
8 10e7 - 10e8
9 10e6 - 10e7
10 10e5 - 10e6
11 10e4 - 10e5
12 10e4 - 10e5

Please note that DNA sample quality also plays a role in the resulting titer. DNA with high levels of contaminants may yield lower titer than those expected for your particular vector.

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My vectors don't have fluorescent markers. What titers should I expect?

By ucsf_admin on July 27, 2020

 

Our preps with vectors smaller than 8 kb yield 107 infectious units per mL. Vectors approximately 12 kb in size yield 105 infectious units per mL.

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Do you titer all virus preps?

By ucsf_admin on July 27, 2020

 

Titration requires that your submitted vector DNA contains a fluorescent marker. If your vectors do not have fluorescent markers, they will not be titered.

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How to you measure viral titer?

By ucsf_admin on July 27, 2020

 

Viral titer is quantified by flow cytometry in units of infectious particles per milliliter (IU/mL). We infect adherent 293T cells and measure their expression of fluorescent markers as a readout for titer.

  • Read more about How to you measure viral titer?
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