My vectors don't have fluorescent markers. What titers should I expect?
Our preps with vectors smaller than 8 kb yield 107 infectious units per mL. Vectors approximately 12 kb in size yield 105 infectious units per mL.
Do you titer all virus preps?
Titration requires that your submitted vector DNA contains a fluorescent marker. If your vectors do not have fluorescent markers, they will not be titered.
How to you measure viral titer?
Viral titer is quantified by flow cytometry in units of infectious particles per milliliter (IU/mL). We infect adherent 293T cells and measure their expression of fluorescent markers as a readout for titer.