Here is a list of frequently asked questions the core commonly receives from virus requesters.
If you have a question and do not see it already answered below, please post it in "e-office" and we will try to get back to you as soon as possible.
Q: Where is the core located?
A: Medical Sciences building S1126 (door code: 774000), on the Parnassus Campus.
Q: Where do I drop off plasmids?
A: In the white fridge to the right side of the door in S1126. There is a black rack in the freezer labeled 'Viracore Drop Off".
Q: How long does it take to complete my virus order?
A: Around 2 weeks from the date of transfection.
Q: What is the virus production schedule like?
A: The core makes viruses twice a week, on Tuesday and Friday. Orders are processed in the order in which they are received.
Q: What is the desired concentration of my plasmid DNA?
A: The plasmid DNA should be at a concentration of 100ng/ul. The cleanest DNA produces the highest quality virus. If you are having difficulty obtaining the concentration, please contact the core.
Q: How much DNA should I bring to the core?
A: The core requires 16ug/6ug/1ug for mega/medi/mini lenti order.
Q: Do you titer every well of the small-scale lentiviral preps (6-well, 12-well, 24-well plates)?
A: No, for "mini", "micro", and "96-well" preps only one well will be titered/plate which will serve as an estimate for the titer for each sample in the plate.
This page will be updated on a regular basis.