Frequently asked questions

Here is a list of frequently asked questions the core commonly receives from virus requesters.

If you have a question and do not see it already answered below, please post it in "e-office" and we will try to get back to you as soon as possible.


Q: Where is the core located?

A: Medical Sciences building S1126 (door code: 774000), on the Parnassus Campus.


Q: Where do I drop off plasmids?

A: In the white fridge to the right side of the door in S1126. There is a black rack in the freezer labeled 'Viracore Drop Off".


Q: How long does it take to complete my virus order?

A: Around 2 weeks from the date of transfection.


Q: What is the virus production schedule like?

A: The core makes viruses twice a week, on Tuesday and Friday. Orders are processed in the order in which they are received.


Q: What is the desired concentration of my plasmid DNA?

A: The plasmid DNA should be at a concentration of 100ng/ul.  The cleanest DNA produces the highest quality virus.  If you are having difficulty obtaining the concentration, please contact the core.


Q: How much DNA should I bring to the core?

A: The core requires 16ug/6ug/1ug for mega/medi/mini lenti order.


Q: Do you titer every well of the small-scale lentiviral preps (6-well, 12-well, 24-well plates)? 

A: No, for "mini", "micro", and "96-well" preps only one well will be titered/plate which will serve as an estimate for the titer for each sample in the plate.



This page will be updated on a regular basis.