BIOSAFETY CONSIDERATIONS AND STANDARD OPERATING PROTOCOLS
Because of the importance of maintaining a safe working environment for investigators, the Core facility is staffed with trained personnel who instruct and oversee the daily operations and use of the Core and its equipment. New investigators planning to utilize any of the Core's facilities are required to undergo training. In addition, investigators wishing to store or obtain reagents from the Core facility may do so.
We are happy to help advise you on lab safety and we implore you to communicate with your local biosafety officer. The ViraCore BUA is attached below, along with additional documents that may be a useful when drafting your own BUA. Again, consult with your local biosafety officer, since all labs using these materials must be approved by the biosafety office. To learn more, visit the NIH RAC Lentiviral Guidance and check out the Guidance attachment below.
We may also post additional protocols on this site. Check out the UCSF Procedures for Using Lentivirus or Adenovirus in Rodents at the ABSL2 Viral Shedding Facility page Lentiviral Replication Safety Assay and a JOVE video on lentivirus production. We also include a protocol for shRNA cloning into lentivirus, and its subsequent use in infection in mouse brain tissue: Lentiviral Strategies for RNAi Knockdown of Neuronal Genes. If you need protocols for lentiviral zygotic injection, contact the Core and we can help. If you have protocols or technical improvements that you would like us to share with the community, let us know.
We've also attached a few files below- including our Core BUA and other relevant biosafety guidelines. We hope they are helpful to you!